Journal: Annals of Translational Medicine

Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway

doi: 10.21037/atm.2020.02.14

Figure Lengend Snippet: Primary antibodies with indicated concentration for WB, IHC, IF

Article Snippet: β-action , 1:800 , , , Bioss , bsm-33036M.

Techniques: Concentration Assay

Journal: Nucleic Acids Research

Article Title: Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway

doi: 10.1093/nar/gkab445

Figure Lengend Snippet: Functional impairment of cell migration suppression for NKILA lacking CAR-N. ( A ) Wound healing in cell migration assay in MCF-7 cells treated with TGF-β. ( B ) Quantification of cell migration at the indicated time points relative to 0 h ( n = 3), *** P < 0.001, ** P < 0.01. ( C ) Western blot showing the relative expression of E-cadherin and vimentin; β-actin is used as the loading control.

Article Snippet: Antibodies targeted to SRSF1 (ProteinTech 12929–2-AP), SRSF7 (ProteinTech 11044-1-AP), DHX9 (ProteinTech 17721-1-AP), HNRNPC (ProteinTech 11760-I-AP), PRPF8 (ProteinTech 11171-I-AP), SRSF9 (ProteinTech 17926-I-AP), U2AF2 (Santa Cruz-48804), E-cadherin (CST-14472), vimentin (ProteinTech 10366–1-AP), and β-actin (Origene-TA811000), all used at a 1:1000 dilution, and to tubulin (Sigma T9026; 1:10 000 dilution), were used for western blotting.

Techniques: Functional Assay, Migration, Cell Migration Assay, Western Blot, Expressing

Journal: The Journal of Biological Chemistry

Article Title: A cathelicidin antimicrobial peptide from Hydrophis cyanocinctus inhibits Zika virus infection by downregulating expression of a viral entry factor

doi: 10.1016/j.jbc.2022.102471

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal anti-β-actin , Abmart , Catalog no.: T40104 , WB (1:5000 dilution).

Techniques: Flow Cytometry, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

Journal: Vaccines

Article Title: Riboflavin as a Mucosal Adjuvant for Nasal Influenza Vaccine

doi: 10.3390/vaccines9111296

Figure Lengend Snippet: Riboflavin promotes DC maturation via the MAPK pathway. ( A – D ) DCs were incubated with riboflavin (40 µM) for 1 h, and then the expressions of p-ERK, ERK, p-P38, P38, p-JNK, JNK, and β-actin were detected by Western blot. ( E – J ) DCs were incubated with FR 180204 (ERK inhibitor, 10 µM), BIRB 796 (P38 MAPK inhibitor, 10 µM), or SP600125 (JNK inhibitor, 10 µM) for 2 h, followed by riboflavin for 24 h. The expressions of MHCII ( E , I ) and CD69 ( F ,J) in DCs were detected via FCM, and then the secretions of IL-12p70 ( G ) and IL-10 ( H ) in the supernatant were detected by ELISA. Results are from one representative experiment out of three performed. All of the data are presented as the mean ± SD. Statistical significance was assessed by unpaired Student’s two-sided t -tests or one-way ANOVA analysis to compare the results between the two groups or different groups, respectively; * p < 0.05, ** p < 0.01.

Article Snippet: Mouse anti-β-actin was obtained from TransGen Biotech (Beijing, China).

Techniques: Incubation, Western Blot, Enzyme-linked Immunosorbent Assay